研究業績

*: corresponding author
#: equally contributed

2021年から

Spatial and single-cell colocalisation analysis reveals MDK-mediated immunosuppressive environment with regulatory T cells in colorectal carcinogenesis.

Hashimoto M, Kojima Y, Sakamoto T*, Ozato Y, Nakano Y, Abe T, Hosoda K, Saito H, Higuchi S, Hisamatsu Y, Toshima T, Yonemura Y, Masuda T, Hata T, Nagayama S, Kagawa K, Goto Y, Utou M, Gamachi A, Imamura K, Kuze Y, Zenkoh J, Suzuki A, Takahashi K, Niida A, Hirose H, Hayashi S, Koseki J, Fukuchi S, Murakami K, Yoshizumi T, Kadomatsu K, Tobo T, Oda Y, Uemura M, Eguchi H, Doki Y, Mori M, Oshima M, Shibata T, Suzuki Y, Shimamura T*, Mimori K*.
EBioMedicine. 103, 105102, 2024. 


IGSF3 is a homophilic cell adhesion molecule that drives lung metastasis of melanoma by promoting adhesion to vascular endothelium.

Guo Y, Kasai Y, Tanaka Y, Ohashi-Kumagai Y, Sakamoto T, Ito T*, Murakami Y*.
Cancer Sci.  in press. 


Mint3-depletion-induced energy stress sensitizes triple-negative breast cancer to chemotherapy via HSF1 inactivation.

Tanaka N#, Okada H#, Yamaguchi K, Seki M, Matsubara D, Gotoh N, Suzuki Y, Furukawa Y, Yamashita T, Inoue J, Kaneko S, Sakamoto T*.
Cell Death Dis. 14, 815, 2023.
 

MT1-MMP as a Key Regulator of Metastasis.

Tanaka N*, Sakamoto T*.
Cells. 12, 2187, 2023. 

RAS遺伝子を中心としたがん治療戦略の確立

田中 伯享*坂本 毅治 

生体の科学 Vol. 74 No.4 p289-294, 2023. 


マクロファージにおける酸素センシング機構調節分子Mint3 の炎症性疾患・がんでの役割

坂本 毅治*, 田中 伯享, 植松 崇之 
サイトメトリーリサーチ 32 巻 2 号 p. 1-9, 2023.
 

L-2hydroxyglutaric acid rewires amino acid metabolism in colorectal cancer via the mTOR-ATF4 axis.

Tabata S*, Kojima Y, Sakamoto T, Igarashi K, Umetsu K, Ishikawa T, Hirayama A, Kajino-Sakamoto R, Sakamoto N, Yasumoto KI, Okano K, Suzuki Y, Yachida S, Aoki M, Soga T*.
Oncogene. 42, 1294-1307, 2023.

Mint3 as a Potential Target for Cooling Down HIF-1-Mediated Inflammation and Cancer Aggressiveness.

Tanaka N, Sakamoto T*.
Biomedicines. 11, 549, 2023.


Hypoxia inducible factor-1 activator munc-18-interacting protein 3 promotes tumour progression in urothelial carcinoma.

Ikeda J#, Ohe C#,Tanaka N#, Yoshida T, Saito R, Atsumi N, Kobayashi T, Kinoshita H, Tsuta K, Sakamoto T*
Clin Transl Discov. 3, e158, 2023.

Spatial and single-cell transcriptomics decipher the cellular environment containing HLA-G+ cancer cells and SPP1+ macrophages in colorectal cancer.

Ozato Y#, Kojima Y#, Kobayashi Y, Hisamatsu Y, Toshima T, Yonemura Y, Masuda T, Kagawa K, Goto Y, Utou M, Fukunaga M, Gamachi A, Imamura K, Kuze Y, Zenkoh J, Suzuki A, Niida A, Hirose H, Hayashi S, Koseki J, Oki E, Fukuchi S, Murakami K, Tobo T, Nagayama S, Uemura M, Sakamoto T, Oshima M, Doki Y, Eguchi H, Mori M, Iwasaki T, Oda Y, Shibata T, Suzuki Y, Shimamura T*, Mimori K*.
Cell Rep. 42, 111929, 2023.

Involvement of membrane palmitoylated protein 2 (MPP2) in the synaptic molecular complex at the mouse cerebellar glomerulus.

Yamada T, Saitoh Y, Kametani K, Kamijo A, Sakamoto T, Terada N*.
Histochem Cell Biol. 158, 497-511, 2022.

Trans-homophilic interaction of CADM1 promotes organ infiltration of T-cell lymphoma by adhesion to vascular endothelium.

Kasai Y, Gan SP, Funaki T, Ohashi-Kumagai Y, Tominaga M, Shiu SJ, Suzuki D, Matsubara D, Sakamoto T, Sakurai-Yageta M, Ito T, Murakami Y*.
Cancer Sci.  113, 1669–1678, 2022. 

CHIP-associated mutant ASXL1 in blood cells promotes solid tumor progression.

Liu X, Sato N, Shimosato Y, Wang TW, Denda T, Chang YH, Yabushita T, Fujino T, Asada S, Tanaka Y, Fukuyama T, Enomoto Y, Ota Y, Sakamoto T, Kitamura T, Goyama S*.
Cancer Sci. 113, 1182-1194, 2022.


Structural and thermodynamical insights into the binding and inhibition of FIH-1 by the N-terminal disordered region of Mint3.

Ten T, Nagatoishi S*, Maeda R, Hoshino M, Nakayama Y, Seiki M, Sakamoto T, Tsumoto K*.

J Biol Chem. 297, 101304, 2021. 


Pharmacological inhibition of Mint3 attenuates tumour growth, metastasis, and endotoxic shock.

Sakamoto T*, Fukui, Kondoh Y, Honda K, Shimizu T, Hara T, Hayashi T, Saitoh Y, Murakami Y, Inoue J, Kaneko S, Osada H, Seiki M.
Commun Biol. 4, 1165, 2021.


TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers. 

Nakasuka F, Tabata S*, Sakamoto T, Hirayama A, Ebi H, Yamada T, Umetsu K, Ohishi M, Ueno A, Goto H, Sugimoto M, Nishioka Y, Yamada Y, Tomita M, Sasaki AT, Yano S, Soga T*.
Commun Biol. 4, 782, 2021.

Mint3 depletion-mediated glycolytic and oxidative alterations promote pyroptosis and prevent the spread of Listeria monocytogenes infection in macrophages. 

Uematsu T, Tsuchiya K, Kobayashi N, Seiki M, Inoue JI, Kaneko S, Sakamoto T*.
Cell Death Dis. 12, 404, 2021.

MMPを標的としたがん微小環境改変治療法の可能性

坂本毅治 

実験医学増刊 Vol.39 No.12 「がん微小環境に1細胞レベルで挑む」 羊土社, 2021.


炎症による転移の制御

坂本毅治

月刊「細胞」2021年 2月号 ニューサイエンス社,p12-15, 2021.




2020年まで


Mint3 is dispensable for pancreatic and kidney functions in mice. 

Chung Y, Saitoh Y, Hayashi T, Fukui Y, Terada N, Seiki M, Murakami Y, Sakamoto T*.
Biochem Biophys Rep. 24, 100872, 2020. 

Generation of a p16 reporter mouse and its use to characterize and target p16high cells in vivo. 

Omori S, Wang T, Johmura Y*, Kanai T, Nakano Y, Kido T, Susaki EA, Nakajima T, Shichino S, Ueha S, Ozawa M, Yokote K, Kumamoto S, Nishiyama A, Sakamoto T, Yamaguchi K, Hatakeyama S, Shimizu E, Katayama K, Yamada Y, Yamazaki S, Miyoshi C, Yanagisawa M, Ueno H, Imoto S, Furukawa Y, Yoshida N, Matsushima K, Ueda HR, Miyajima A, Nakanishi M*.
Cell Metab. 32, 814-828, 2020.
 

Mint3 depletion restricts tumor malignancy of pancreatic cancer cells by decreasing SKP2 expression via HIF-1. 

Kanamori A, Matsubara D, Saitoh Y, Fukui Y, Gotoh N, Kaneko S, Seiki M, Murakami Y, Inoue J, Sakamoto T*.
Oncogene. 39, 6218-6230, 2020.

 

EXOSC9 depletion attenuates P-body formation, stress resistance, and tumorigenicity of cancer cells. 

Yoshino S, Matsui Y, Fukui Y, Seki M, Yamaguchi K, Kanamori A, Saitoh Y, Shimamura T, Suzuki Y, Furukawa Y, Kaneko S, Seiki M, Murakami Y, Inoue JI, Sakamoto T*.
Sci Rep. 10, 9275, 2020.
 

Mint3 mitigates renal fibrosis after ischemia-reperfusion injury through protection of tubular epithelial cells from apoptosis via up-regulation of NF-κB. 

Nasu K, Kawakami T, Shinohara A, Sakamoto T, Nangaku M*.
Nephrol Dial Transplant. 35, 576-586, 2020.

Electron microscopic observation of photoreceptor cells in directly inserted anesthetized Drosophila into a high-pressure freezing unit.

Terada H, Saitoh Y, Kametani K, Sakaguchi M, Sakamoto T, Kamijo A, Terada N*.
Microsc Res Tech. 82, 244-249, 2019.

Scaffold protein Lin7 family in membrane skeletal protein complex in mouse seminiferous tubules. 

Kamijo A, Saitoh Y, Sakamoto T, Kubota H, Yamauchi J, Terada N*
Histochem Cell Biol. 152, 333-343, 2019.
 

The membrane palmitoylated protein, MPP6, is involved in myelin formation in the mouse peripheral nervous system. 

Saitoh Y, Kamijo A, Yamauchi J, Sakamoto T, Terada N*.
Histochem Cell Biol. 151, 385-394, 2019.

Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. 

Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, Kuchimaru T, Sakamoto T, Watanabe H, Kondoh G, Shinae Kizaka-Kondoh*. 
Oncotarget. 9, 11209-11226, 2018.

Deficiency of a membrane skeletal protein, 4.1G, results in myelin abnormalities in the peripheral nervous system. 

Saitoh Y, Ohno N, Yamauchi J, Sakamoto T, Terada N*.
Histochem Cell Biol. 148:597-606, 2017.
 

Mint3 in bone marrow-derived cells promotes lung metastasis in breast cancer model mice. 

Hara T, Murakami Y, Seiki M, Sakamoto T*.
Biochem Biophys Res Commun. 490, 688-692, 2017.

Control of metastatic niche formation by targeting APBA3/Mint3 in inflammatory monocytes. 

Hara T, Nakaoka HJ, Hayashi T, Mimura K, Hoshino D, Inoue M, Nagamura F, Murakami Y, Seiki M, Sakamoto T*.
Proc Natl Acad Sci U S A. 114:E4416-E4424, 2017.
 

Mint3-mediated L1CAM expression in fibroblasts promotes cancer cell proliferation via integrin α5β1 and tumour growth. 

Nakaoka HJ, Tanei Z, Hara T, Weng JS, Kanamori A, Hayashi T, Sato H, Orimo A, Otsuji K, Tada K, Morikawa T, Sasaki T, Fukayama M, Seiki M, Murakami Y, Sakamoto T*.  
Oncogenesis. 6, e334, 2017.

Integrated functions of membrane-type 1 matrix metalloproteinase in regulating cancer malignancy: beyond a proteinase.

Sakamoto T*, Seiki M*.
Cancer Sci. 108, 1095-1100, 2017. (Review)

Mint3/Apba3 depletion ameliorates severe murine influenza pneumonia and macrophage cytokine production in response to the influenza virus. 

Uematsu T, Fujita T, Nakaoka HJ, Hara T, Kobayashi N, Murakami Y, Seiki M, Sakamoto T*. 
Sci Rep. 6:37815, 2016.

Mint3 potentiates TLR3/4- and RIG-I-induced IFN-β expression and antiviral immune responses. 

Huai W, Song H, Yu Z, Wang W, Han L, Sakamoto T, Seiki M, Zhang L, Zhang Q, Zhao W*. 
Proc Natl Acad Sci U S A. 113, 11925-11930, 2016.
 

The ERK-signaling target RNF126 regulates anoikis resistance in cancer cells by changing the mitochondrial metabolic flux. 

Yoshino S, Hara T, Nakaoka HJ, Kanamori A, Murakami Y, Seiki M, Sakamoto T*. 
Cell Discov. 2, 16019, 2016.

NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells.

Nakaoka HJ, Hara T, Yoshino S, Kanamori A, Matsui Y, Shimamura T, Sato H, Murakami Y, Seiki M, Sakamoto T*. 
Sci Rep. 6, 22784, 2016.
 

Investigation of a MMP-2 Activity-Dependent Anchoring Probe for Nuclear Imaging of Cancer. 

Temma T, Hanaoka H, Yonezawa A, Kondo N, Sano K, Sakamoto T, Seiki M, Ono M, Saji H*. 
PLoS One. 9, e102180, 2014.
 

Hypoxia-Inducible Factor 1 Regulation through Cross Talk between mTOR and MT1-MMP. 

Sakamoto T, Weng JS, Hara T, Yoshino S, Kozuka-Hata H, Oyama M, Seiki M*. 
Mol Cell Biol. 34, 30-42, 2014. 
 

MT1-MMP plays a critical role in hematopoiesis by regulating HIF-mediated chemokine/cytokine gene transcription within niche cells. 

Nishida C, Kusubata K, Tashiro Y, Gritli I, Sato A, Ohki-Koizumi M, Morita Y, Nagano M, Sakamoto T, Koshikawa N, Kuchimaru T, Kizaka-Kondoh S, Seiki M, Nakauchi H, Heissig B, Hattori K*. 
Blood. 119, 5405-16, 2012.
 

Genetic screening of new genes responsible for cellular adaptation to hypoxia using a genome-wide shRNA library. 

Yoshino S, Hara T, Weng JS, Takahashi Y, Seiki M*, Sakamoto T.
PLoS One. 7, e35590, 2012.
 

Genetic dissection of proteolytic and non-proteolytic contributions of MT1-MMP to macrophage invasion.

Hara T, Mimura K, Seiki M*, Sakamoto T.
Biochem Biophys Res Commun. 413, 277-281, 2011.
 

Deletion of the Mint3/APBA3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccaride-induced septic shock. 

Hara T, Mimura K, Abe T, Shioi G, Seiki M*, Sakamoto T
J Biol Chem. 286, 32542-32551, 2011.
 

Targeting the warburg effect, which arises in tumor cells expressing membrane-type-1 matrix metalloproteinase. 

Sakamoto T, Niiya D, Seiki M*. 
J Biol Chem. 286, 14691-704, 2011. 
 

ZF21 is a new regulator of focal adhesion disassembly and a potential member of the spreading initiation center. 

Nagano M, Hoshino D, Sakamoto T, Akizawa T, Koshikawa N, Seiki M*. 
Cell Adh Migr. 5, 23-28, 2011.
 

A membrane protease regulates energy production in macrophages by activating hypoxia-inducible factor-1 via a non-proteolytic mechanism. 

Sakamoto T, Seiki M*.
J Biol Chem. 285, 29951-64, 2010.
 

ZF21 protein regulates cell adhesion and motility. 

Nagano M, Hoshino D, Sakamoto T, Kawasaki N, Koshikawa N, Seiki M*.
J Biol Chem. 285, 21013-22, 2010.
 

Mint3 enhances the activity of hypoxia-inducible factor-1 (HIF-1) in macrophages by suppressing the activity of factor inhibiting HIF-1. 

Sakamoto T, Seiki M*. 
J Biol Chem. 284, 30350-9, 2009. 

 

Identification and characterization of Lutheran blood group glycoprotein as a new substrate of membrane-type 1 matrix metalloproteinase 1 (MT1-MMP): a systemic whole cell analysis of MT1-MMP-associating proteins in A431 cells. 

Niiya D, Egawa N, Sakamoto T, Kikkawa Y, Shinkawa T, Isobe T, Koshikawa N, Seiki M*. 
J Biol Chem. 284, 27360-9, 2009.
 

Cytoplasmic tail of MT1-MMP regulates macrophage motility independently from its protease activity. 

Sakamoto T, Seiki M*. 
Genes Cells. 14, 617-26, 2009.
 

Establishment of an MT4-MMP-deficient mouse strain representing an efficient tracking system for MT4-MMP/MMP-17 expression in vivo using beta-galactosidase. 

Rikimaru A, Komori K, Sakamoto T, Ichise H, Yoshida N, Yana I, Seiki M*. 
Genes Cells. 12, 1091-100, 2007.
 

Stroma-derived matrix metalloproteinase (MMP)-2 promotes membrane type 1-MMP-dependent tumor growth in mice. 

Taniwaki K, Fukamachi H, Komori K, Ohtake Y, Nonaka T, Sakamoto T, Shiomi T, Okada Y, Itoh T, Itohara S, Seiki M, Yana I*.
Cancer Res. 67, 4311-9, 2007.
 

Developmental expression and localization of IA-2 mRNA in mouse neuroendocrine tissues. 

Shimizu S, Saito N, Kubosaki A, SungWook S, Takeyama N, Sakamoto T, Matsumoto Y, Saeki K, Matsumoto Y, Onodera T*.
Biochem Biophys Res Commun. 288, 165-71, 2001.